The innovative Biphenyl is Restek's most popular LC stationary phase because it is particularly adept at separating compounds that are hard to resolve or that elute early on C18 and other phenyl chemistries. As a result, the rugged Raptor Biphenyl column is extremely useful for fast separations in bioanalytical testing applications like drug and metabolite analyses, especially those that require a mass spectrometer (MS). Increasing retention of early-eluting compounds can limit ionization suppression, and the heightened selectivity helps eliminate the need for complex mobile phases that are not well suited for MS detection.

Stationary Phase Category: Phenyl (L11)
Ligand Type: Biphenyl
Particle: 1.8 µm, 2.7 µm, or 5 µm superficially porous particle (SPP or “core-shell” particle) silica
Pore Size: 90 Å
Carbon Load: 7% (1.8 µm); 7% (2.7 μm); 5% (5 μm)
End-Cap: yes
Surface Area: 125 m²/g (1.8 µm); 130 m²/g (2.7 µm); or 100 m²/g (5 µm)
Recommended Usage:
pH Range: 2.0 to 8.0
Maximum Temperature: 80 °C
Maximum Pressure: 1034 bar/15,000 psi* (1.8 μm); 600 bar/8700 psi (2.7 μm); 400 bar/5800 psi (5 μm)

* For maximum lifetime, recommended maximum pressure for 1.8 μm particles is 830 bar/12,000 psi.

Properties:

• Increased retention for dipolar, unsaturated, or conjugated solutes.
• Enhanced selectivity when used with methanolic mobile phase.
• Ideal for increasing sensitivity and selectivity in LC-MS analyses.

Switch to a Biphenyl column when:

• Limited selectivity is observed on a C18.
• You need to increase retention of hydrophilic aromatics.

Similar Phases

Supelco/Millipore Sigma: Ascentis Express Biphenyl (2.7 µm)

Phenomenex: Kinetex Biphenyl