miRNeasy FFPE Kit is intended for purification of microRNA and total RNA from formalin-fixed, paraffin-embedded tissue sections in molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The miRNeasy FFPE Kit enables purification of total RNA that includes RNA from approximately 18 nucleotides upwards from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The kit provides recovery of usable RNA fragments, including miRNA and other small RNAs, for applications such as quantitative, real-time RT-PCR. Procedures can be automated on the QIAcube Connect MDx.
Yields and performance of total RNA and miRNA with the miRNeasy FFPE Kit are superior to alternative methods of miRNA purification, such as using phenol-chloroform extraction or a similar kit from another supplier
The entire miRNeasy FFPE procedure can be completed in as little as 85 minutes. The optimized lysis buffer allows sample lysis with proteinase K digestion in only 15 minutes without sacrificing RNA yields. After lysis, samples are incubated at 80ºC for 15 minutes to reverse formalin crosslinking. Genomic DNA is then rapidly removed in an optimized DNase step with DNase Booster Buffer, and concentrated RNA is purified using RNeasy MinElute spin columns. Since RNA is eluted in a volume of just 14–30 µl, smaller reaction volumes are possible in downstream applications.
|Applications||PCR, qPRC, real-time RT-PCR, microArray|
|Elution volume||14-30 µl|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||miRNA, total RNA|
|Sample amount||up to 4 sections, each with a thickness up to 10 µm and a surface area up to 250mm^2 (automated protocol on QIAcube: up to 2 sections)|
|Processing||Manual (protocol for automated processing on QIAcube available)|
|Main sample type||FFPE tissue samples|