Qiagen

QIAamp DNA Blood Mini Kit (250)

QIAamp DNA Blood Mini Kit is intended for for purification of genomic, mitochondrial or viral DNA from blood and other body fluids in molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Includes: QIAGEN® QIAamp DNA Blood Mini Kit, 250 DNA minipreps

description

QIAamp DNA Blood Mini Kit provides silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. The kits are designed for a range of sample sizes from 200 μl up to 10 ml fresh or frozen human whole blood. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. A dedicated kit is also available for automated purification of 1–12 samples on the QIAcube Connect MDx.

Performance

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples. The purified DNA is suitable for long-range PCR amplification and restriction fragment length polymorphism (RFLP) analysis used, for example, for paternity testing.

The QIAamp DNA Blood Midi Kit yields up to 94.5% recovery of DNA; the QIAamp DNA Blood Maxi Kit yields up to 95.8% recovery of DNA, depending on the starting cell densities.

Procedure

QIAamp DNA Blood Kits simplify DNA purification from blood and DNA purification from body fluids with fast spin-column, vacuum, centrifugation or automated procedures. Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA and heparin, may be processed. If processing QIAamp Midi or Maxi spin columns on vacuum manifolds additional Buffer AW1 [cat. no. 19081] and Buffer AW2 [cat. no. 19072] are required for use with the vacuum manifolds.

Vacuum processing with the QIAamp DNA Blood Mini Kit

With the QIAamp DNA Blood Mini Kit, blood can be processed by vacuum instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.

Automated processing on the QIAcube Connect

The award-winning QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated – up to 12 samples can be processed per run. The QIAcube Connect used together with the dedicated QIAamp DNA Mini QIAcube Kit provides a winning combination for fast, easy and convenient DNA purification.

Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit.

QIAamp DNA Blood technology yields genomic, mitochondrial or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

Applications

QIAamp DNA Blood Kits provide proven QIAamp technology for the purification of DNA from a variety of materials. Sample sources include:

Fresh and frozen whole blood or buffy coat
Plasma or serum
Bone marrow
Lymphocytes
Platelets
Body fluids
Cultured cells
Swabs and buccal cells

DNA yields from human whole blood with different cell densities

Leukocytes per ml	DNA yield (µg)		DNA recovery (%)
	QIAamp Midi	QIAamp Maxi	QIAamp Midi	QIAamp Maxi
2.5 x 10⁵	3.0	15.8	92.3	95.8
1.0 x 10⁶	11.0	60.4	91.7	91.5
5.0 x 10⁶	62.4	312.0	94.5	94.5
1.0 x 10⁷	116.3	624.6	88.1	94.6

FEATURES & BENEFITS

Rapid purification of high-quality, ready-to-use DNA
No organic extraction or alcohol precipitation
Consistent, high yields
Complete removal of contaminants and inhibitors for reliable results
Kit formats for low- to high-throughput – options for automation of all kits