Qiagen

QIAamp Viral RNA Mini Kit (250)

QIAamp Viral RNA Mini Kit is intended for viral RNA isolation from cell-free body fluids in molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Includes: QIAGEN® QIAamp Viral RNA Mini Kit, 250 RNA preps

description

QIAamp Viral RNA Mini Kit simplifies viral RNA purification from cell-free body fluids with fast spin-column, vacuum and plate centrifugation procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube Connect MDx using the QIAamp Viral RNA Mini Kit or dedicated QIAamp Viral RNA Mini QIAcube Kit.

QIAamp Viral RNA Mini standard protocol can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through QIAamp Viral RNA protocol while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.

Performance

The high-quality viral RNA isolated using the QIAamp Viral RNA Mini Kit performs well in a wide range of downstream applications, including viral genotyping, viral epidemiology and infectious disease research.

The QIAamp 96 Viral RNA Kit has been successfully used to recover RNA from viruses, including SARS-CoV-2 virus. Performance of the QIAamp 96 Viral RNA Kit is comparable to that achieved with the QIAamp Viral RNA Mini Kit. RNA isolation is proven from commonly used saliva collection and stabilization methods and no cross-contamination was detected

Procedure

Optimized buffers and enzymes lyse samples, stabilize nucleic acids and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column or 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.

Principle

QIAamp RNA technology yields viral RNA from cell-free body fluids ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.

Applications

QIAamp Viral RNA Kits simplify viral RNA purification from cell-free body fluids, offering fast spin-column, vacuum or plate centrifugation procedures, or automation on QIAcube. Sample types include:

Plasma and serum
CSF
Urine
Other cell-free body fluids
Cell-culture supernatants
Swabs

Comparison of QIAamp Viral RNA Kits

Features	QIAamp Viral RNA Mini Kit	QIAamp 96 Viral RNA Kit
Applications	PCR, qPCR, real-time PCR	PCR, qPCR, real-time PCR
Elution volume	50 µl	80 µl
Format	Spin columns	96-well plate
Main sample type	Liquid media	Liquid media
Processing	Manual (centrifugation or vacuum)	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A mRNA, DNA or protein	Viral RNA	Viral RNA
Sample amount	140 µl	140 µl solution from a swab sample
Technology	Silica technology	Silica technology
Time per run or per prep	20–40 minutes
Yield	>90% recovery

FEATURES & BENEFITS

Rapid isolation of high-quality, ready-to-use RNA
No organic extraction or alcohol precipitation
Complete removal of contaminants and inhibitors
RNA isolation from viruses, such as SARS-CoV-2, up to 96-well format
Automated purification from low to ultra-high throughput format