• Contact Us
  • About Us
  • Log In or Create an Account
Menu
  • Contact Us
  • About Us
  • Log In or Create an Account

We’re here to help.

  • Toll Free: 866-205-9215
  • info@completelabsystems.com
×
$0.00 0 Cart
  • COVID-19 TESTS
  • CLIA Waived Rapid Tests
    • Drugs of Abuse
    • Alcohol Testing
    • Drugs of Abuse ID Kits
    • Physician Office Lab
  • RT-PCR & Molecular
    • Automated Extraction
    • Manual Extraction
    • RT-PCR Analyzers
    • RT-PCR Panels & Reagents
  • CLIA Laboratory
    • Allergy
    • Chemistry
    • Hematology
    • Immunoassay
    • Toxicology
    • Open Channel Chemistry Reagents
    • General Chemistry Controls
  • Lab Consumables
    • Centrifuges & Tubes
      • Centrifuges
      • Centrifuge Tubes
    • ESR Products
    • Freezers & Refrigerators
    • Fume Hoods
    • Pipettes & Tips
      • Pipettes
      • Pipette Tips
    • Plates & Consumables
      • Extraction
      • RT-PCR
      • ELISA Plates
    • Sample Collection & Transport
      • Transport Medium
      • Swabs
      • Specimen Bags
    • Test Tubes & Tube Racks
      • Test Tubes - Plastic
      • Test Tubes - Glass
      • Tube Racks
Home / RT-PCR & Molecular Diagnostics / Manual Extraction
QIAamp Viral RNA Mini Kit by Qiagen

Qiagen

QIAamp Viral RNA Mini Kit (250)

QIAamp Viral RNA Mini Kit is intended for viral RNA isolation from cell-free body fluids in molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

REQUEST QUOTE
INCLUDES
QIAGEN® QIAamp Viral RNA Mini Kit, 250 RNA preps
 
 

description

QIAamp Viral RNA Mini Kit simplifies viral RNA purification from cell-free body fluids with fast spin-column, vacuum and plate centrifugation procedures. Viral RNA binds specifically to the QIAamp silica membrane, and pure viral RNA is eluted in either water or a buffer provided with the kit. Purification can be fully automated on the QIAcube Connect MDx using the QIAamp Viral RNA Mini Kit or dedicated QIAamp Viral RNA Mini QIAcube Kit.

QIAamp Viral RNA Mini standard protocol can also be executed using the TRACKMAN Connected system, paired with PIPETMAN M Connected pipettes, both from Gilson. The TRACKMAN Connected system guides researchers through QIAamp Viral RNA protocol while automatically adjusting the Bluetooth-enabled PIPETMAN M Connected pipette settings. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report.

Performance

The high-quality viral RNA isolated using the QIAamp Viral RNA Mini Kit performs well in a wide range of downstream applications, including viral genotyping, viral epidemiology and infectious disease research.

The QIAamp 96 Viral RNA Kit has been successfully used to recover RNA from viruses, including SARS-CoV-2 virus. Performance of the QIAamp 96 Viral RNA Kit is comparable to that achieved with the QIAamp Viral RNA Mini Kit. RNA isolation is proven from commonly used saliva collection and stabilization methods and no cross-contamination was detected

Procedure

Optimized buffers and enzymes lyse samples, stabilize nucleic acids and enhance selective RNA adsorption to the QIAamp membrane. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. Alcohol is added and lysates loaded onto the QIAamp spin column or 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use RNA is then eluted in water or low-salt buffer.

Principle

QIAamp RNA technology yields viral RNA from cell-free body fluids ready to use in RT-PCR and blotting procedures. QIAamp sample preparation technology is fully licensed.

No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in either water or a buffer provided with the kit.

Applications

QIAamp Viral RNA Kits simplify viral RNA purification from cell-free body fluids, offering fast spin-column, vacuum or plate centrifugation procedures, or automation on QIAcube. Sample types include:

  • Plasma and serum
  • CSF
  • Urine
  • Other cell-free body fluids
  • Cell-culture supernatants
  • Swabs

Comparison of QIAamp Viral RNA Kits

FeaturesQIAamp Viral RNA Mini KitQIAamp 96 Viral RNA Kit
ApplicationsPCR, qPCR, real-time PCRPCR, qPCR, real-time PCR
Elution volume50 µl80 µl
FormatSpin columns96-well plate
Main sample typeLiquid mediaLiquid media
ProcessingManual (centrifugation or vacuum)Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A mRNA, DNA or proteinViral RNAViral RNA
Sample amount140 µl140 µl solution from a swab sample
TechnologySilica technologySilica technology
Time per run or per prep20–40 minutes
Yield>90% recovery

FEATURES & BENEFITS

  • Rapid isolation of high-quality, ready-to-use RNA
  • No organic extraction or alcohol precipitation
  • Complete removal of contaminants and inhibitors
  • RNA isolation from viruses, such as SARS-CoV-2, up to 96-well format
  • Automated purification from low to ultra-high throughput format
 
  • Manufacturer Catalog #: 52906
  • SKU: CLS52906
  • Categories: Manual Extraction

More Products

miRNeasy FFPE Kit by Qiagen

Manual Extraction

miRNeasy FFPE Kit (50)

QIASymphony SP by QiagenQiagen QIASymphony SP

Automated Extraction

QIAsymphony SP

QIAcube Connect MDx by QiagenQIACube Connect MDx by Qiagen

Automated Extraction

QIAcube Connect MDx

Omega BioTek

Manual Extraction

Omega BioTek: E.Z.N.A.® Universal Pathogen DNA Kit

COMPANY
  • About Us
  • Blog/News
  • Contact Us

CLIA WAIVED RAPID TESTS

  • Alcohol Testing
  • Drugs of Abuse Screening
  • Drugs of Abuse ID Kits
  • Physician Office Lab

CLIA LABORATORY

  • Clinical Lab
  • Toxicology
  • Molecular
  • Lab Consumables
CONTACT US
  • Local: 818-205-9221
  • Toll Free: 866-205-9215
  • info@completelabsystems.com

6320 Canoga Ave. Ste. 1500, FL 15
Woodland Hills, CA 91367

STAY CONNECTED
Facebook-f Twitter Youtube

SIGN UP FOR OUR NEWSLETTER

Terms and Conditions
Privacy Policy
Cookies
Do Not Sell My Info
Copyright © 2023 – Complete Laboratory Systems. All rights reserved.
    • COVID-19 TESTS
    • CLIA Waived Rapid Tests
      • Drugs of Abuse
      • Alcohol Testing
      • Drugs of Abuse ID Kits
      • Physician Office Lab
    • RT-PCR & Molecular
      • Automated Extraction
      • Manual Extraction
      • RT-PCR Analyzers
      • RT-PCR Panels & Reagents
    • CLIA Laboratory
      • Allergy
      • Chemistry
      • Hematology
      • Immunoassay
      • Toxicology
      • Open Channel Chemistry Reagents
      • General Chemistry Controls
    • Lab Consumables
      • Centrifuges & Tubes
        • Centrifuges
        • Centrifuge Tubes
      • ESR Products
      • Freezers & Refrigerators
      • Fume Hoods
      • Pipettes & Tips
        • Pipettes
        • Pipette Tips
      • Plates & Consumables
        • Extraction
        • RT-PCR
        • ELISA Plates
      • Sample Collection & Transport
        • Transport Medium
        • Swabs
        • Specimen Bags
      • Test Tubes & Tube Racks
        • Test Tubes - Plastic
        • Test Tubes - Glass
        • Tube Racks
  • Login
  • Newsletter

This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Read More

Accept

Cookie Settings

  • About Cookies

    About Cookies

    Cookies are small text files that can be used by websites to make a user's experience more efficient. The law states that we can store cookies on your device if they are strictly necessary for the operation of this site. For all other types of cookies we need your permission. This site uses different types of cookies. Some cookies are placed by third party services that appear on our pages.
  • Necessary

    Necessary

    Always Active
    Necessary cookies help make a website usable by enabling basic functions like page navigation and access to secure areas of the website. The website cannot function properly without these cookies.
    NameDomainPurposeExpiryType
    wpl_user_preference completelabsystems.com WP GDPR Cookie Consent Preferences 1 year HTTP
  • Marketing

    Marketing

    Marketing cookies are used to track visitors across websites. The intention is to display ads that are relevant and engaging for the individual user and thereby more valuable for publishers and third party advertisers.
  • Analytics

    Analytics

    Analytics cookies help website owners to understand how visitors interact with websites by collecting and reporting information anonymously.
    NameDomainPurposeExpiryType
    _gid completelabsystems.com Google Universal Analytics short-time unique user tracking identifier. 1 days HTTP
    _ga completelabsystems.com Google Universal Analytics long-time unique user tracking identifier. 2 years HTTP
  • Preferences

    Preferences

    Preference cookies enable a website to remember information that changes the way the website behaves or looks, like your preferred language or the region that you are in.
  • Unclassified

    Unclassified

    Unclassified cookies are cookies that we are in the process of classifying, together with the providers of individual cookies.
    NameDomainPurposeExpiryType
    _gat_gtag_UA_212460542_1 completelabsystems.com --- Session ---
    _ga_9YYXEQ0TN4 completelabsystems.com --- 2 years ---

Login

Lost your password?

Register

A password will be sent to your email address.

Your personal data will be used to support your experience throughout this website, to manage access to your account, and for other purposes described in our privacy policy.

Request Quote

  • This field is for validation purposes and should be left unchanged.