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Qiagen RNeasy FFPE Kit is intended for purification of total RNA from formalin-fixed, paraffin-embedded tissue sections in molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The RNeasy FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions reverse formaldehye modification of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase and DNase Booster Buffer for optimized removal of genomic DNA contamination. RNeasy MinElute spin columns enable purification of total RNA with elution volumes of as low as 10 μl. Purification can be fully automated on the QIAcube Connect MDx.
With the RNeasy FFPE Kit, yields of RNA purified from fixed and embedded samples are greater than those achieved using other methods. Recovered RNA is intact down to 70 nucleotides in length and virtually free of genomic DNA contamination. Usable RNA fragments purified with the kit from FFPE samples are highly suited for use in sensitive downstream applications, including real-time RT-PCR.
The entire RNeasy FFPE procedure can be completed in as little as 85 minutes. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. DNase treatment then effectively removes genomic DNA, including small DNA fragments. Finally, concentrated RNA is purified using RNeasy MinElute spin columns, and eluted in a volume of 14–30 µl. Purification can be fully automated on the QIAcube.
|Applications||PCR, qPCR, real-time RT-PCR, microarray|
|Elution volume||14–30 µl|
|Main sample type||FFPE tissue samples|
|Processing||Manual (centrifugation), automated (QIAcube)|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||RNA|
|Time per run or per prep||70 minutes|
|Sample amount||1*5 µm to 4*10µm sections|